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产品分类
AffiniPure Goat anti-mouse IgGsIgM (Peroxidase Horseradish
AffiniPure Goat anti-mouse IgGsIgM (Peroxidase Horseradish
物品单位 品牌
毫升 Frdbio
  • 产地:中国
  • 货号:SAB(9010△6)H
  • 发布日期: 2020-07-29
  • 更新日期: 2020-07-30
产品详细说明
产地 中国
品牌 Frdbio
货号 SAB(9010△6)H
用途范围
纯度 %
CAS编号
规格 ul
是否进口

Catalog No.:SAB(90106)H

Target: Mouse

Host: Goat

Format: Whole IgG

Specificity: mouse IgG except IgM, Fcγ Fragment Specific

NoCross Reactivity: Human, Bovine, Rabbit Serum Proteins

Conjugate: Horseradish Peroxidase

Clonality: Polyclonal

Purity:Antigen specific purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Buffer:50% glycerol 0.01M PBS,pH 7.6

Stabilizer:14.5 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: None

Physical State: Liquid

Storage & Expiration Date-20°C. for 24months

Suggested Working Concentration or Dilution Range:

1:500 -1:5,000 for immunohisto/cytochemistry.

1:5,000-1:100,000 for ELISA and Western blotting with chromogenic substrates
1:10,000 - 1:200,000 for Western blotting with ECL substrates

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be

determined empirically.

本产品基于间接ELISA实验测试,排除与小鼠IgM外的其他小鼠IgG亚型的非Fab部分反应,与血清中非免疫球蛋白的其他组分没有交叉反应;经过测试,本产品与人,牛和兔血清蛋白没有任何交叉反应,不排除与其他物种的血清有交叉反应的可能(没有测试其他物种数据)。

本二抗产品最显著的用途是用于间接ELISA方法进行非IgM亚型单克隆抗体细胞株筛选:在单克隆抗体中如果筛选到IgM亚型的抗体非常棘手,本产品将IgM亚型单克隆抗体细胞株扼杀在摇篮中,基本原理是本二抗不会针对IgM亚型产生反应,因此筛选的时候得到的细胞株全部为非IgM亚型。

 

 

*本试剂仅供实验室研究使用